| Product Name | Calretinin - ELISA | | Catalog-No. | EA611/96 | | Description | Enzyme Immuno Assay | | Format | 12x8 Determinations, Microtiter plate, single break apart wells; coated with polyclonal calretinin antibodies | | Procedure | Sample volume: 15 µl patient serum or plasma
1. Sample Preparation: 60 min shaking at room temperature
2. ELISA: 5 hours incubation at room temperature
Measurement in ELISA reader at 450 nm (620 nm reference wavelength)
6 Standards with range: 0.25 ng/ml - 4.0 ng/ml
2 Kit controls, lyophilized
Enzyme Conjugate 200 x concentrated; Wash buffer 50 x concentrated
All other reagents ready for use | | Clinical Fields | Oncology, cancer screening, occupational diseases | | Diseases | Mesothelioma | | Normal Value Specificity | see instruction for use | | Sensitivity | 0.05 ng/ml | | Characteristics | Sandwich ELISA with microtiter plate formate
Highly specific and sensitive rabbit anti-calretinin antibody
Good linearity between 0.25 - 4.0 ng/ml
Easy to handle, total assay time is 6 hours
Suitable for testing large series of serum or plasma samples
Validated in larger case-control studies
Involved in prospective studies |
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