Zymolyase-20T / Zymolyase-100T - 维百奥（北京）生物科技有限公司

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产品名称 Zymolyase-20T / Zymolyase-100T

货号：

07663-91/07663-04；07665-55/07665-84

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产品描述

产品名称：Zymolyase-20T / Zymolyase-100T

品牌：Nacalai

Zymolyase.pdf

Zymolyase^®, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase^® is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit

Product Information

There are two preparations of Zymolyase^®, Zymolyase®-20T and Zymolyase^®-100T, having lytic activity of 20,000 units/g and 100,000 units/g respectively. Zymolyase^®-20T is ammonium sulfate precipitate while Zymolyase^®-100T is a further purified preparation by affinity chromatography⁽⁹⁾. Lytic activity varies depending on yeast strain, growth stage of yeast, or cultural conditions^(6-8). Further informations related to Zymolyase^® can be obtained in the reference section below^(12-16).

Product Name		Zymolyase^®-20T	Zymolyase^®-100T
Form		Lyophilized Powder
Purification		Ammonium Sulfate Precipitation	Affinity Chromatography
Activity		20,000 units/g	100,000 unites/g
Essential enzyme		β-1,3-glucan laminaripentaohydrolase
Other activities contained (※1)	β-1,3-glucanase	approx. 1.5 x 10⁶ units/g	approx. 1.0 x 10⁷ units/g
	protease	approx. 1.0 x 10⁴ units/g	approx. 1.7 x 10⁴ units/g
	mannanase	approx. 1.0 x 10⁶ units/g	approx. 6.0 x 10⁴ units/g
Contaminants	Amylase	Trace amount	Non detectable
	Xylanase
	Phosphatase
Optimum pH and Temp.		pH7.5, 35°C (for lysis of viable yeast cells)
		pH6.5, 45°C (for hydrolysis of yeast glucan)
Stability	2°C	No loss of activity was found after storage for 1 year
Heat stability	30°C	70% of the lytic activity is lost after storage for 3 months
		90% of the lytic activity is lost after storage for 3 months
	60°C	Lytic activity is lost on incubation for 5 minutes
Specificity (Lytic Spectrum)		Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, kluyveromyces, Lipomyces, Metschnikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schwanniomyces, etc.

(※1) See reference, Kitamura, K., Kaneko, T., Yamamoto, Y., J. Gen. Appl. Microbiol., 18, 57 (1972) as to the definition of each enzyme units.

Ordering Information

Product Name	Storage	Product No.	PKG Size	Price
Zymolyase^®-20T	R	07663-91	1 g	请咨询客服
		07663-04	10 g
Zymolyase^®-100T	R	07665-55	500 mg	请咨询客服
		07665-84	5 g

Zymolyase® is a registered trademark of Kirin Holdings Company, Limited

(Storage) R: Refrigerator

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