A negative control antibody used to distinguish specific signal from background noise.

Role in R&D/Diagnostics:

● Baseline Setting: An isotype control is an antibody with the same immunoglobulin class (e.g., IgG1, IgM) and from the same host species as the primary antibody, but with no known specificity for the target antigen.

● Identifying Non-Specific Binding: It reveals the level of background signal caused by factors like Fc receptor binding on cells, electrostatic interactions, or non-specific protein adsorption. Any signal above the isotype control indicates specific binding of the primary antibody.

● Critical for Validation: In flow cytometry and immunoassays, using an isotype control is essential for accurately setting gates and thresholds, ensuring that positive results are true positives, not artifacts. This is a fundamental part of diagnostic assay validation and quality control.

Vicbio Biotechnology Co., Ltd. is an authorized distributor of Nebulabio. Please find the product list below: